- Cultivation of a GMO for heterologous expression of a cytochrome P450 monooxygenase
- Induction of the expression of the target enzyme
- Purification of monooxygenase using various affinity techniques
- Characterization of the purified enzyme
- Basic balancing of the process
- Simulation and scaling of the process
Module MV-BioVT-214-M-7
Bioprocess Engineering Lab II (M, 3.0 LP)
Module Identification
Module Number | Module Name | CP (Effort) |
---|---|---|
MV-BioVT-214-M-7 | Bioprocess Engineering Lab II | 3.0 CP (90 h) |
Basedata
CP, Effort | 3.0 CP = 90 h |
---|---|
Position of the semester | 1 Sem. in WiSe |
Level | [7] Master (Advanced) |
Language | [DE] German |
Module Manager | |
Lecturers | |
Area of study | [MV-BioVT] Bioprocess Engineering |
Reference course of study | [MV-88.805-SG] M.Sc. Biological Process Engineering |
Livecycle-State | [NORM] Active |
Courses
Type/SWS | Course Number | Title | Choice in Module-Part | Presence-Time / Self-Study | SL | SL is required for exa. | PL | CP | Sem. | |
---|---|---|---|---|---|---|---|---|---|---|
3L | MV-BioVT-86441-K-7 | Bioprocess Engineering Lab II
| P | 42 h | 48 h |
L-Schein
| - | no | 3.0 | WiSe |
- About [MV-BioVT-86441-K-7]: Title: "Bioprocess Engineering Lab II"; Presence-Time: 42 h; Self-Study: 48 h
- About [MV-BioVT-86441-K-7]: The study achievement [L-Schein] proof of successful participation in the practical course / lab must be obtained.
- About [MV-BioVT-86441-K-7]:
Depending on the examination regulations, the course work can/must be submitted as graded examination work. In this case the repetition rules of the examination regulations for practical laboratory work apply.
Contents
Competencies / intended learning achievements
The students are able
- to explain basic principles of heterologous expression of proteins, biotechnological processes independently,
- to apply affinity techniques for the purification of recombinant proteins,
- to perform process simulation
- to present and discuss their results in the laboratory group
- to present their results in the form of an experimental protocol and discuss them in detail
Literature
- Script for the internship
- W. Storhas; Bioverfahrensentwicklung; Wiley-VCH (2003) ISBN 3-527-28866-X
- H. Chmiel; Bioprozesstechnik; Elsevier ISBN 3-8247-1607-8
Requirements for attendance (informal)
Recommended requirements: In-depth knowledge of bioprocess engineering, knowledge of microbiology
Modules:
Requirements for attendance (formal)
Proof of participation in the safety training according to the German Ordinance on Hazardous Substances, not more than one year ago.
References to Module / Module Number [MV-BioVT-214-M-7]
Course of Study | Section | Choice/Obligation |
---|---|---|
[MV-88.A29-SG] M.Sc. Biological and Chemical Engineering | Studienschwerpunkt I | [WP] Compulsory Elective |
Module-Pool | Name | |
[MV-ALL-MPOOL-6] | Wahlpflichtmodule allgemein | |
[MV-BioVT-MPOOL-6] | Wahlpflichtmodule Bioverfahrenstechnik |
Notes on the module handbook of the department Mechanical and Process Engineering
Ausnahmen: