Module Handbook

  • Dynamischer Default-Fachbereich geändert auf MV

Notes on the module handbook of the department Mechanical and Process Engineering

Die hier dargestellten veröffentlichten Studiengang-, Modul- und Kursdaten des Fachbereichs Maschinenbau und Verfahrenstechnik ersetzen die Modulbeschreibungen im KIS und wuden mit Ausnahme folgender Studiengänge am 28.10.2020 verabschiedet.


Course MV-BioVT-86441-K-7

Bioprocess Engineering Lab II (3L, 3.0 LP)

Course Type

SWS Type Course Form CP (Effort) Presence-Time / Self-Study
3 L Laboratory course 3.0 CP 42 h 48 h
(3L) 3.0 CP 42 h 48 h


CP, Effort 3.0 CP = 90 h
Position of the semester 1 Sem. in WiSe
Level [7] Master (Advanced)
Language [DE] German
Area of study [MV-BioVT] Bioprocess Engineering
Additional informations
Livecycle-State [NORM] Active

Possible Study achievement

  • Verification of study performance: practical laboratory / experimental work
  • Examination number (Study achievement): 10441 ("Bioprocess Engineering Lab II")
  • Details of the examination (type, duration, criteria) will be announced at the beginning of the course.
    Passing the entrance colloquium, carrying out all experiments in the laboratory and writing a protocol (proof of active participation in the laboratory), weighting 30% colloquium; 70% protocol.

    Depending on the examination regulations, the course work can/must be submitted as graded examination work. In this case the repetition rules of the examination regulations for practical laboratory work apply.


  • Cultivation of a GMO for heterologous expression of a cytochrome P450 monooxygenase
  • Induction of the expression of the target enzyme
  • Purification of monooxygenase using various affinity techniques
  • Characterization of the purified enzyme
  • Basic balancing of the process
  • Simulation and scaling of the process

Competencies / intended learning achievements

The students are able
  • to explain basic principles of heterologous expression of proteins, biotechnological processes independently,
  • to apply affinity techniques for the purification of recombinant proteins,
  • to perform process simulation
  • to present and discuss their results in the laboratory group
  • to present their results in the form of an experimental protocol and discuss them in detail


  • Script for the internship
  • W. Storhas; Bioverfahrensentwicklung; Wiley-VCH (2003) ISBN 3-527-28866-X
  • H. Chmiel; Bioprozesstechnik; Elsevier ISBN 3-8247-1607-8


Script for the lab is available on the BioVT homepage. For further information and course materials please consider the corresponding OLAT-course.

Requirements for attendance (informal)

Recommended requirements: In-depth knowledge of bioprocess engineering, knowledge of microbiology


Requirements for attendance (formal)

Proof of participation in the safety training according to the German Ordinance on Hazardous Substances, not more than one year ago.

References to Course [MV-BioVT-86441-K-7]

Module Name Context
[MV-BioVT-214-M-7] Bioprocess Engineering Lab II P: Obligatory 3L, 3.0 LP
[MV-BioVT-M103-M-7] Bioprocess Engineering II P: Obligatory 3L, 3.0 LP