Module Handbook

  • Dynamischer Default-Fachbereich geändert auf MV

Notes on the module handbook of the department Mechanical and Process Engineering

Die hier dargestellten veröffentlichten Studiengang-, Modul- und Kursdaten des Fachbereichs Maschinenbau und Verfahrenstechnik ersetzen die Modulbeschreibungen im KIS und wuden mit Ausnahme folgender Studiengänge am 28.10.2020 verabschiedet.

Ausnahmen:

Course MV-BioVT-86441-K-7

Bioprocess Engineering Lab II (3L, 3.0 LP)

Course Type

SWS Type Course Form CP (Effort) Presence-Time / Self-Study
3 L Laboratory course 3.0 CP 42 h 48 h
(3L) 3.0 CP 42 h 48 h

Basedata

SWS 3L
CP, Effort 3.0 CP = 90 h
Position of the semester 1 Sem. in WiSe
Level [7] Master (Advanced)
Language [DE] German
Lecturers
Area of study [MV-BioVT] Bioprocess Engineering
Additional informations
Livecycle-State [NORM] Active

Possible Study achievement

  • Verification of study performance: practical laboratory / experimental work
  • Examination number (Study achievement): 10441 ("Bioprocess Engineering Lab II")
  • Details of the examination (type, duration, criteria) will be announced at the beginning of the course.
    Passing the entrance colloquium, carrying out all experiments in the laboratory and writing a protocol (proof of active participation in the laboratory), weighting 30% colloquium; 70% protocol.

    Depending on the examination regulations, the course work can/must be submitted as graded examination work. In this case the repetition rules of the examination regulations for practical laboratory work apply.


Contents

  • Cultivation of a GMO for heterologous expression of a cytochrome P450 monooxygenase
  • Induction of the expression of the target enzyme
  • Purification of monooxygenase using various affinity techniques
  • Characterization of the purified enzyme
  • Basic balancing of the process
  • Simulation and scaling of the process

Competencies / intended learning achievements

The students are able
  • to explain basic principles of heterologous expression of proteins, biotechnological processes independently,
  • to apply affinity techniques for the purification of recombinant proteins,
  • to perform process simulation
  • to present and discuss their results in the laboratory group
  • to present their results in the form of an experimental protocol and discuss them in detail

Literature

  • Script for the internship
  • W. Storhas; Bioverfahrensentwicklung; Wiley-VCH (2003) ISBN 3-527-28866-X
  • H. Chmiel; Bioprozesstechnik; Elsevier ISBN 3-8247-1607-8

Materials

Script for the lab is available on the BioVT homepage. For further information and course materials please consider the corresponding OLAT-course.

Requirements for attendance (informal)

Recommended requirements: In-depth knowledge of bioprocess engineering, knowledge of microbiology

Modules:

Requirements for attendance (formal)

Proof of participation in the safety training according to the German Ordinance on Hazardous Substances, not more than one year ago.

References to Course [MV-BioVT-86441-K-7]

Module Name Context
[MV-BioVT-214-M-7] Bioprocess Engineering Lab II P: Obligatory 3L, 3.0 LP
[MV-BioVT-M103-M-7] Bioprocess Engineering II P: Obligatory 3L, 3.0 LP