Module Handbook

  • Dynamischer Default-Fachbereich geändert auf MV

Notes on the module handbook of the department Mechanical and Process Engineering

Die hier dargestellten veröffentlichten Studiengang-, Modul- und Kursdaten des Fachbereichs Maschinenbau und Verfahrenstechnik ersetzen die Modulbeschreibungen im KIS und wuden mit Ausnahme folgender Studiengänge am 28.10.2020 verabschiedet.

Ausnahmen:

Course MV-BioVT-86440-K-4

Bioprocess Engineering Lab I (3L, 3.0 LP)

Course Type

SWS Type Course Form CP (Effort) Presence-Time / Self-Study
3 L Laboratory course 3.0 CP 42 h 48 h
(3L) 3.0 CP 42 h 48 h

Basedata

SWS 3L
CP, Effort 3.0 CP = 90 h
Position of the semester 1 Sem. in SuSe
Level [4] Bachelor (Specialization)
Language [DE] German
Lecturers
Area of study [MV-BioVT] Bioprocess Engineering
Additional informations
Livecycle-State [NORM] Active

Possible Study achievement

  • Verification of study performance: practical laboratory / experimental work
  • Examination number (Study achievement): 10440 ("Bioprocess engineering I (lab)")
  • Details of the examination (type, duration, criteria) will be announced at the beginning of the course.
    Entry colloquium to the laboratory & graded protocol (weighting 30% colloquium; 70% protocol)

    Depending on the examination regulations, the course work can/must be submitted as graded examination work. In this case the repetition rules of the examination regulations for practical laboratory work apply.


Contents

Block I: Microbiological basics

This practical part serves as an introduction to the work with microorganisms and the necessary equipment.

  • Production of solid and liquid media
  • Sterile working under the Cleanbench
  • Cultivation of microorganisms in shaking flasks
  • Microscopy and cell counting
  • Determination of biomass via bio dry mass or by photometer

Block II: Fermentation

In this block the operation and control of a fermenter and the supervision of a cultivation should be practiced. Thereby, operating parameters of the fermenter will be adjusted and a cell culture from pre-culture to cell harvest will be performed.

  • Preparation of a cultivation (autoclaving, inoculation etc.)
  • Operating parameters for a submerged cultivation (pH-value, T, kla-value)
  • Monitoring the growth of microorganisms (bio-dry matter, metabolism)

Block III: Reconditioning

The aim of the experiment is to isolate an enzyme (e.g. alcohol dehydrogenase) from a microorganism (e.g. Saccharomyces cerevisiae) using various purification steps. Starting from the raw extract of the cell disruption, different methods are applied, each successively increasing the purity of the enzyme to be isolated.

  • Cell Digestion
  • Heat denaturation of proteins
  • Salting out proteins
  • Chromatographic protein purification
  • Affinity chromatography

Block IV: Biotransformation

The aim of biotransformation is the determination of the Michaelis constant and the maximum reaction rate of an enzyme (e.g. of lactate dehydrogenase from rabbit muscle) and the inhibition of enzyme activity by various inhibitors (e.g. oxamate and reduced nicotinamide adenine dinucleotide (NADH+H+)).

  • Conversion of substrates by biocatalysts
  • Analysis of kinetic data

Competencies / intended learning achievements

The students are able
  • to clarify the necessary working methods in a laboratory and apply them to experiments
  • to perform cell cultivation from pre-culture to cell harvest
  • to obtain enzymes in a targeted manner by the use of microorganisms
  • to evaluate data on the reaction rate and to make statements on enzyme activity.

Literature

  • Script for the internship
  • W. Storhas; Bioverfahrensentwicklung; Wiley-VCH (2003) ISBN 3-527-28866-X
  • H. Chmiel; Bioprozesstechnik; Elsevier ISBN 3-8247-1607-8

Materials

Print and digital media. For further information and course materials please consider the corresponding OLAT-course.

Requirements for attendance (informal)

Knowledge in microbiology or biotechnology

Modules:

Requirements for attendance (formal)

None

References to Course [MV-BioVT-86440-K-4]

Module Name Context
[MV-BioVT-77-M-4] Bioprocess Engineering Lab I P: Obligatory 3L, 3.0 LP