Module Handbook

  • Dynamischer Default-Fachbereich geändert auf MV

Notes on the module handbook of the department Mechanical and Process Engineering

Die hier dargestellten veröffentlichten Studiengang-, Modul- und Kursdaten des Fachbereichs Maschinenbau und Verfahrenstechnik ersetzen die Modulbeschreibungen im KIS und wuden mit Ausnahme folgender Studiengänge am 28.10.2020 verabschiedet.

Ausnahmen:

Course MV-BIOVT-86442-K-7

Bioanalytics Lab (3L, 3.0 LP)

Course Type

SWS Type Course Form CP (Effort) Presence-Time / Self-Study
3 L Laboratory course 3.0 CP 42 h 48 h
(3L) 3.0 CP 42 h 48 h

Basedata

SWS 3L
CP, Effort 3.0 CP = 90 h
Position of the semester 1 Sem. in WiSe
Level [7] Master (Advanced)
Language [DE] German
Lecturers
Area of study [MV-BioVT] Bioprocess Engineering
Additional informations
Livecycle-State [NORM] Active

Possible Study achievement

  • Verification of study performance: practical laboratory / experimental work
  • Examination number (Study achievement): 10442 ("Bioanalyties Lab")
  • Details of the examination (type, duration, criteria) will be announced at the beginning of the course.
    Passing the entrance colloquium, carrying out all experiments in the laboratory and writing a protocol (weighting 30% colloquium; 70% protocol)

    Depending on the examination regulations, the course work can/must be submitted as graded examination work. In this case the repetition rules of the examination regulations for practical laboratory work apply.


Contents

Using the example of a microbial fermentation (e.g. E. coli or S. cerevisiae), different sensor systems are investigated in case of disturbances of the bioprocess:
  • pH and T measurement in the bioreactor
  • Dissolved gas and exhaust gas analysis
  • Cell count determination

In addition, methods for product analysis are used:

  • Determination of the glucose concentration by means of biosensor technology
  • Determination of protein content (staining methods)
  • 2D gel electrophoresis
  • 3D fluorescence spectroscopy
  • Capillary Electrophoresis

Competencies / intended learning achievements

The students are able
  • to apply bioanalytical systems in practice, which have so far only been taught in theory during the course of studies
  • to carry out necessary analyses on bioprocesses independently
  • to evaluate the achieved measurement results in order to derive process control strategies
  • to present and discuss their results in the laboratory group

Literature

  • F. Lottspeich, J.W. Engels; Bioanalytik; Elsevier ISBN-10: 3-8274-1520-9
  • Script for the internship

Materials

Print media, script for the laboratory available on the BioVT homepage. For further information and course materials please consider the corresponding OLAT-course.

Requirements for attendance (informal)

In-depth knowledge in the execution of fermentations (bacteria and fungi)

Courses

Requirements for attendance (formal)

Proof of participation in the safety training according to the German Ordinance on Hazardous Substances, not more than one year ago.

References to Course [MV-BIOVT-86442-K-7]

Module Name Context
[MV-BioVT-128-M-7] Bioanalytics Lab P: Obligatory 3L, 3.0 LP