Module Handbook

  • Dynamischer Default-Fachbereich geändert auf BIO

Course BIO-MGE-01-K-7

Molecular Genetics II (L, 12.0 LP)

Course Type

SWS Type Course Form CP (Effort) Presence-Time / Self-Study
- L Laboratory course 12.0 CP 160 h 200 h
(L) 12.0 CP 160 h 200 h

Basedata

SWS L
CP, Effort 12.0 CP = 360 h
Position of the semester 1 Sem. in WiSe
Level [7] Master (Advanced)
Language [EN] English
Lecturers
Area of study [BIO-MGE] Molecular Genetics
Livecycle-State [NORM] Active

Contents

Mammalian cell cultures as a model system, working under sterile conditions, preparation of buffers and media, basic techniques for culturing of human cell lines, site-directed mutagenesis, construction of a vector carrying a mutated gene, plasmid purification, DNA sequencing, cell transfection, cell growth analysis, quantitative and qualitative analysis of cellular response to different stress factors such as DNA damage agents or overexpression of mutant protein, RNAi, flow cytometry, preparation of cell lysates, SDS-PAGE, Western blot, quantification of complex biological data, use of online databases such as PubMed and NCBI, BLAST searches and sequence alignments, presentation of scientific data, basics of scientific writing; good scientific practice.

Literature

Will be provided during the orientation meeting.

Requirements for attendance (informal)

None

Requirements for attendance (formal)

Passed examination of [BIO-MGE-01-K-5] "Genetics, Genomics and Epigenetics"

References to Course [BIO-MGE-01-K-7]

Course-Pool Name
[BIO-VM_MCB-KPOOL-7] Advanced Practical MCB
[BIO-VM_MPBiotech-KPOOL-7] Advanced Practical MPBiotec