Module Handbook

  • Dynamischer Default-Fachbereich geändert auf BIO

Course BIO-BTE-01-K-5

Mass Spectrometry and Deep sequencing Based Strategies in Systems Biology (S, 3.0 LP)

Course Type

SWS Type Course Form CP (Effort) Presence-Time / Self-Study
- S Reading course 3.0 CP 24 h 66 h
(S) 3.0 CP 24 h 66 h


CP, Effort 3.0 CP = 90 h
Position of the semester 1 Sem. in WiSe
Level [5] Master (Entry Level)
Language [EN] English
Area of study [BIO-BTE] Molecular Biotechnology and Systems Biology
Livecycle-State [NORM] Active

Possible Study achievement

  • Verification of study performance: continuous and active participation in the courses
  • Details of the examination (type, duration, criteria) will be announced at the beginning of the course.


The students will hear introductory lectures, read research and review papers, and discuss together and with the teacher the following topics:

− Hardware and device

− Deep sequencing techniques

− Quantitative proteomics strategies

− Experimental design using deep sequencing technology and quantitative proteomics

Competencies / intended learning achievements

Professional competence:

− Understanding the molecular basis of current next generation sequencing (NGS) methods.

− Knowledge on how mass analyzers work and how they are implemented in modern mass spectrometers

− Understanding how peptide sequences can be identified from fragment spectra and how protein abundance can be estimated from mass spectra

− Gaining fundamental knowlege of the different approaches and experimental basics to use deep sequencing and MS-based methods

Methodological competence:

Social competence:

− Learning to discuss scientific issues with other students and the teacher and explaining complex concepts

− Learning to simplify complicated topics and ideas and to identify their key aspects


− Acquirement of scientific knowledge by self-responsible reading of English research literature and identification of aspects that require further expllanation

− Critical evaluation of current research data

Intended Learning Outcomes:

On successfully completing the module students will be able to…

− understand the molecular and technological basis of NGS and MS-based methods.

− read and understand English literature on Systems Biology approaches.

− read and discuss research papers orally


Bioanalytik, F. Lottspeich, J.W. Engels 2012, Springer Spektrum; original/review articles will be provided at or prior to the beginning of the course


PDFs of lecture and literature for seminar will be provided on the homepage of the department Molecular Biotechnology & Systems Biology

Requirements for attendance (informal)

Bachelor-grade knowledge in Molecular Biotechnology, Genetics, Biochemistry, and Cell Biology

Requirements for attendance (formal)

Admission to the Master Program ‘Biology’

References to Course [BIO-BTE-01-K-5]

Course-Pool Name
[BIO-LR_all-KPOOL-5] Lecture & Reading Course - all
[BIO-LR_MCB-KPOOL-5] Lecture & Reading Course: MCB
[BIO-LR_MPBiotech-KPOOL-5] Lecture & Reading Course: MPBiotech